Volume No. 10 Issue No.: 1 Page No.: 14-20 July-September 2015

 

EXPLORATION OF THE HEMATINIC ACTIVITY AND TOXICITY OF A TRADITIONAL AYURVEDIC MEDICINAL FORMULATION FOR TREATMENT OF ANAEMIA USING RAT MODEL

 

Vishal S. Khelukar1and Aniroodha V. Pethkar2*

1. Life Insurance Corporation of India, Fort, Mumbai, Maharashtra (INDIA) 2. Department of Biotechnology, Government Institute of Science, Nipatniranjan Nagar, Caves Road, Aurangabad, Maharashtra (INDIA)

 

Received on : March 15, 2015

 

ABSTRACT

 

The ancient Indian ayurvedic medicinal formulations are getting increasing attention due to the health benefits, minimal adverse effects and environment friendliness. Numerous researchers have attempted the exploration of traditional and plant-based medicinal preparations using bio-chemical analyses and in-vitro experimentation. The present study highlights the in-vivo therapeutic efficacy of the ayurvedic formulation- navyas louha, a combination of plant extracts and inorganic iron for treatment of anaemia. Anaemia was experimentally induced in Wistar rats by administering desferrioxamine (1/10th LD50 value) via intramuscular route. The test formulation was administered per-oral route for specified duration of time along with positive control (ferrous succinate) and negative control (dextrose saline). The treatment was continued for 30 days with intermittent testing of animals for various parameters such as body weight, behaviour and the haematological parameters such as hemoglobin, glucose, WBC count, RBC count, MCH, MCV, MCHC, etc. Animals were allowed to recover in the post treatment period as per World Health Organization (WHO) guidelines. It was found that the ayurvedic preparation effectively restored normal haematological and physical body parameters without signs of toxicity. In contrast, conventional therapy consisting of ferrous succinate (positive control) showed altered physiological and body characteristics.

 

Keywords : Ayurvedic, Plant extracts, Anaemia, toxicity, Wistar rats, In-vivo, Nanoparticles

 

 

BACK