Volume No. 9 Issue No.: 4 Page No.: 1081-1087 April-June 2015

 

BIOABSORPTION OF Cd, Pb AND Ni METAL TOLERANT Aspergillus niger AND Penicilium SP. USING SINGLE AND MULTIMETAL SOLUTION

 

Pattanayak B.*, Mittra B. 1 and Dhal N.K. 2

1. Department of Biotechnology, F.M. University, Odisha (INDIA) 2. Department of Environment and Sustainability, CSIR-IMMT, Odisha (INDIA)

 

Received on : January 15, 2015

 

ABSTRACT

 

Cd2+,Pb2+and Ni2+ resistant Fungi including Aspergillus and Penicillium were isolated from waste effluent, collected from the discharge point of Nalco smelter in Kulad, Anugul, district of Odisha, India. Metal tolerance in term of Minimum Inhibitory condition (MIC) was 125- 550?g/ml for Cd ,300-600 ?g/ml for Pb and 300-850 ?g/ml for Ni against tested Fungi. Two isolates, Aspergillus niger and Penicillium sp. were tested for their Cd, Pb and Ni biosorption potential using alkali treated, dried and powdered mycelium. Biosorption experiment was conducted in 100 ml of solution at three initial metal concentrations i.e., 2, 4 and 6 mM with contact time (18 hr) and pretreated fungal biomass (0.1g) at 25C.Biosorption of all metals were round higher at 4 mM, initial metal concentration, as compared to biosorption at 2 and 6 mM concentrations. At 4 mM initial metal concentration Pb biosorption was 18.05 and 19.3 mg/g of Aspergillus and Penicillium biomasses, respectively. Similarly, biosorption of Cd and Ni ions was also maximum at 4 mM initial metal concentration by Aspergillus (19.4 mg/g for Cd and 25.05 mg/g of biomass for Ni) and Penicillium (18.6 mg/g for Cd and 17.9 mg/g of biomass for Ni). In general, biosorption of metal was influenced by initial metal concentration and type of the tested fungi. The results indicated that fungi of metal contaminated soil have high level of metal tolerance and biosorption capacity.

 

Keywords : Filamentous fungi, Metal tolerance, Metal biosorption, Aspergillus, Penicillium

 

 

BACK