Volume No. 9 Issue No.: 3A Page No.: 878-883 January-March 2015

 

PRELIMINARY PHYTOCHEMICAL SCREENING AND In Vitro ANTIMICROBIAL ACTIVITIES OF MEDICINAL PLANT EXTRACTS

 

Minocheherhomji Farida P.*1 and Vyas Bharat M.2

1. Department of Microbiology, B. P. Baria Science Institute, Navsari, Gujarat (INDIA) 2. Department of Health, Ahmedabad Municipal Corporation, Ahmedabad (INDIA)

 

Received on : October 10, 2014

 

ABSTRACT

 

Medicinal plants are used for various ailments by Ayurvedic and Unani pharmacy and also by traditional healers. This study was aimed at carrying out phytochemical analysis and antimicrobial investigation of different plant extracts in water, acetone and methanol. The medicinal plants used for the purpose are Tinospora cordifolia, Withania sominifera, Asparagus racemosus and Oscimum sanctum against a panel of clinically significant bacterial, fungal and yeast strains. Phytochemical screening revealed the presence of alkaloids, tannins, saponins, flavonoids, carbohydrates, protiens, steroids, terpenoids and cardioglycosides. Presence of anthraquinone, anthrocyanins were observed in a few samples where as phenolic flavonoids and phlobatannins were totally absent. Susceptibility by disc diffusion assay revealed significant antimicrobial activity of methanol and acetone extracts of these medicinal plants against gram positive bacteria such as Bacillus subtilis, Staphylococcus aureus and gram negative bacteria such as Proteus vulgaris, Salmonella typhi and Escherichia coli. The extracts obtained in methanol showed marked effect as antimicrobial agent than the extracts obtained in acetone and water

 

Keywords : Antimicrobial activity, Phytochemical constituents, Medicinal plant extracts, Methanol extracts, Gram negative bacteria

 

 

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