Volume No. 9 Issue No.: 3A Page No.: 849-856 January-March 2015

 

EFFECTIVENESS OF IMMOBILIZED SILVER NANOPARTICLES IN INACTIVATION OF PATHOGENIC BACTERIA

 

Bharti S.1,Agnihotri S.2, Mukherji S.3and Mukherji S.1*

1. Centre for Environmental Science and Engineering, IIT, Bombay, Powai, Mumbai(INDIA) 2. Centre for Research in Nanotechnology and Science, IIT, Bombay, Powai, Mumbai(INDIA) 3. Department of Bioscience and Bioengineering, IIT, Bombay, Powai, Mumbai (INDIA)

 

Received on : November 10, 2014

 

ABSTRACT

 

This study involved preparation of a functionalized glass-AgNP nanocomposite. Its antimicrobial effect was tested using a few pathogenic bacteria commonly present in drinking water biofilms i.e., Escherichia coli, Pseudomonas aeruginosa, Burkholderia cepacia and Acinetobacter baumanii. Functionalized glass-AgNP nanocomposite was prepared through immobilization of colloidal AgNPs on a (3-Aminopropyl)-triethoxysilane (APTES) functionalized glass substrate. FEG-SEM confirmed uniform and homogenous distribution of AgNPs on the surface andEDX confirmed significantloading of silver on the surface. AFM also confirmed significant loading of AgNPs on the glass surface showing increase in surface roughness from 0.542 nmto 3.29 nm after AgNP immobilization. Complete disinfection of 100 ml water (103 CFU/ml) was achieved within 60-70 minutes for all the strainsusingthe glassAgNPs nanocomposite (1x1 cm2) in a batch reactor.ICP-AES analysis confirmed minimal leaching of silver in the treated water(<100 ppb, US EPA). This confirms the applicability of this AgNP-immobilized glass substrate as an effective and safeantimicrobial agent for drinking water disinfection

 

Keywords : Biofilm,Disinfection,Immobilization,Leaching,Nanocomposites

 

 

BACK