Volume No. 9 Issue No.: 3A Page No.: 827-832 January-March 2015

 

In vitro MICRO PROPAGATION : A TOOL FOR CONSERVATION OF ENDANGERED AND MEDICIN -ALLY IMPORTANT FOREST TREES

 

Tiwari P

Department of Botany, Plant, Tissue Culture Lab, Shivaji Science College, Nagpur (INDIA)

 

Received on : September 14, 2014

 

ABSTRACT

 

Ecological degradation and its corollary biodiversity loss pose a serious threat to development. Deforestation has resulted in serious damage to biodiversity and gene resources. Medicinal plants play a vital role in the modern economy and India is probably the richest, oldest and most diverse cultural tradition in utilizing medicinal plants. Oroxylum indicum, a popular medicinal tree species in Asia, belongs to family Bignoniaceae, commonly known as shyonal, sopatha is an important ingredient in Ayurvedic preparation called Dasamoola. Due to its medicinal value, indiscriminate exploitation for medicinal purpose coupled with low regeneration and habitat destruction have posed serious threat to its survival and pushed it into list of endangered species in some areas. So the conservation, rapid multiplication and rehabilitation of this species have become immediate need of the country. To overcome this threat the present study has been made to set proper protocol through Plant tissue culture technique using hypocotyl explants of Oroxylum indicum for shoot regeneration. Effect of PGs (Plant Growth hormones) like BAP, NAA and IBA in different conc. and combinations on shoot regeneration was studied. In the present study 5 mg/l and 2.5 mg/l BAP alone and 5 mg/l BAP in combination with 2.5 and 1.0 mg/l NAA was found to be suitable combination for multiple shoot regeneration. Hypocotyl proved to be efficient for multiple shooting. 3% sucrose in MS medium was used. The observation was taken after 20-25 days of incubation. Regenerated shoots were subcultured onto the medium supplemented with different concs. of IBA for rooting. Rooted shoots were successfully grown in pots after acclimatization using vermiculite irrigated with MS salt solution

 

Keywords : Oroxylum indicum, Multiplication, Conservation, Hypocotyl, Plant growth hormones

 

 

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