Volume No. 9 Issue No.: 3A Page No.: 787-796 January-March 2015

 

BIOREMEDIATION OF ACID MINE DRAINAGE : A PROTEOMIC AND GENOMIC APPROACH

 

Sarvamangala H. and Girisha S. T.*

Department of Microbiology and Biotechnology Jnana Bharathi Campus, Bangalore University, Bangalore (INDIA)

 

Received on : November 11, 2014

 

ABSTRACT

 

The most significant environmental issue that has arisen in the mining industries over the last two decades is that of Acid Mine Drainage or Acid Rock Drainage (AMD/ARD) from tailing dumps and waste mined rock piles. Bacillus subtilis and its metabolic products have been used in our studies. In this work, selective separation of pyrite from oxides such as quartz and calcite as well as galena (sulphide mineral) was achieved. Through microbially induced flotation, pyrite could be separated with 90% efficiency from quartz and calcite leading to desulphurisation and depyritization making the process environmentally benign. Mineral specific proteins were found in extracellular proteins isolated from the cell free extract when B. subtilis was grown in presence of minerals through SDSPAGE. The suppression of some genes was observed in the RAPD profile of the genomic DNA in presence of minerals. The cells of B. subtilis proved to be 45-60% efficient in the biosorption of lead metal ions at 10 and 50ppm of lead metal ion. The exopolymers extracted from B. subtilis proved to be 50-60% efficient in biosorption of lead metal ions. This study shows possibilities for development of eco friendly technologies for bioremediation

 

Keywords : Bioremediation, Acid Mine Drainage, Lead, Sulphide, Proteins

 

 

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