Volume No. 9 Issue No.: 3A Page No.: 762-772 January-March 2015

 

EVALUATION OF ANTIMYCOTIC ACTIVITY OF Eucalyptus globulus, Datura stramonium AND Tagetes patula AGAINST THREE ECONOMICALLY IMPORTANT PLANT PATHOGENS

 

Sundari Krishna S.,* Kotiyal Srishti, Singhai Poorva and Gupta Nilanshu

Jaypee Institute of Information Technology, NOIDA, Uttar Pradesh (INDIA)

 

Received on : October 15, 2014

 

ABSTRACT

 

The use of chemical pesticides poses a serious risk to health as well as the environment. Studies showing the hazardous effects of conventional pesticides led to a ban on several pesticides while also indicating the need of exploring unconventional options. Biopesticides have proved to be one such viable option as they are inherently less toxic than conventional pesticides. They are also more target specific, effective in much smaller quantities and quick to decompose leading to lower exposures and lesser pollution problems with high yield. Extracts from plants having biocidal properties can be developed, tested for proliferation of plants pathogens and can further be developed as bio-pesticides. Therefore, we undertook a comparative study of extracts from three plants Eucalyptus globules, Datura stramonium, Tagetes patula along with Azadirachta indica as a control due to its proven biocidal properties. We further chose three fungal plant pathogens (Fusarium oxysporium, Macrophomina phaseolina and Sclerotium rolfsii) that cause considerable economic losses to crop plants to be challenged with the plant extracts. In vitro bioassays (ring test and dry weight analysis) were performed where individual plant pathogen was challenged with a known amount (ppm) of all four biocidal plant extracts respectively. Both from the ring test and dry weight analysis, it was observed that eucalyptus extract has more bio cidalability than neem and was more effective in controlling the growth of both M. phaseolina and F.oxysporium. The difference in biocidal efficiency of the two oils was more than 50% viz. (dry weight of pathogen when challenged with neem was 0.241 gm whereas eucalyptus brought down the dry weight to 0.112gm). There was also marked difference in the extent of growth (measured as radial growth diameter) and sporulation in pathogen when challenged with different plant extracts and eucaluptus gave uniformly better results amongst all four. Our study reports the effectiveness of Tagetes for the first time for its antimycotic nature. Further experiments are underway to determine the dosage and time of exposure of these plant extracts that would help in developing new phyto-biopesticides

 

Keywords : Phyto-biopesticides, Antimycotic, Biocidal, Macrophomina, Fusarium

 

 

BACK