Volume No. 9 Issue No.: 3A Page No.: 755-761 January-March 2015

 

EFFECT OF TEMPERATURE ON HYDROCARBON DEGRADATION ABILITY BY BACTERIAL ISOLATES : A BIOSTIMULATION STUDY

 

Sar Pinaki, *1 Sarkar Jayeeta, Samadder Subhradeep, Velappan Hemalatha1, Roy Ajoy and Kazy Sufia K.2

1. Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal (INDIA) 2. Department of Biotechnology, National Institute of Technology Durgapur, West Bengal (INDIA)

 

Received on : September 29, 2014

 

ABSTRACT

 

Bioremediation of highly contaminated petroleum waste is one of the critical challenges of environmental biotechnology. Nutrient deficiency, especially nitrogen has been reported to be of vital significance affecting rate of hydrocarbon degradation by microorganisms. In this study, we were report a novel method for enrichment of bacteria from oily sludge sample collected from Indian oil cooperation limited refinery Guwahati, Assam, India. Under various nitrogen amendment conditions. In this method, we use multiple nitrogen sources for nitrate, nitrite and ammonium (concentration range of 1-12mM) along with sodium pyrophosphate as dislodging chemical. Further to estimate the biodegradation ability of these isolates effect of temperature (30oC, 40oC, 50oC) on their growth has been studied using aliphatic (pentadecane) and aromatic (naphthalene) compounds as carbon sources. Microcosm based studies on original sludge sample showed a correlation between the three factors are temperature, growth and biodegradability of bacteria with increasing temperature having a detrimental effect on microbial growth. Crucially our findings suggest that response of N amended bacteria to increase in temperature was less drastic as compared to unamended bacteria

 

Keywords : Biostimulation, Temperature, Hydrocarbon, Aliphatic, Aromatic, Naphthalne

 

 

BACK