Volume No. 9 Issue No.: 3 Page No.: 530-540 January-March 2015

 

STUDY OF INDIVIDUAL AND SIMULTANEOUS DEGRADATION OF CHROMIUM (VI) AND PHENOL USING TWO POTENT INDIGENOUS MICROORGANISMS

 

Das Debadatta*1 and Mishra Susmita2

1. Department of Biotechnology & Medical Engineering, National Institute of Technology, Rourkela (INDIA) 2. Department of Chemical Engineering, National Institute of Technology, Rourkela (INDIA)

 

Received on : October 10, 2014

 

ABSTRACT

 

Phenol and chromium are the two major pollutants being discharged in the effluents of various industrial operations. Individual and simultaneous degradation of phenol and chromium was studied in the presence of two indigenous bacterial strain of Pseudomonas putida (MTCC 1194) and Escherichia coli (NCIM 5051) respectively. The optimum conditions for maximum degradation of both the pollutants are temperature 300C, pH 7, inoculum volume 5%, shaking at 130 rpm, with peptone and dextrose as nitrogen and carbon source respectively. At optimized process conditions the Pseudomonas putida (MTCC 1194) effectively degrades a maximum 1000 ppm of phenol in (70 72) hours and Escherichia coli (NCIM 5051) degrades a maximum 40 ppm of Cr(VI) in (40 42) hours. The simultaneous degradation of phenol and chromium by the co-culture of microorganisms is also studied at various phenol and chromium concentration of 500 and 750 ppm of phenol and (5, 10 and 15) ppm of Cr(VI) respectively. The results indicate that the pollutants were successfully reduced to their permissible limits. This is a green method of treatment which produces no harmful by product thus can be considered as an ecofriendly method for the treatment of industrial waste water

 

Keywords : Chromium, Phenol, Biodegradation, Pseudomonas putida, Escherichia coli

 

 

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