Volume No. 9 Issue No.: 2 Page No.: 389-395 October-December 20

 

BIOSYNTHESIS OF NANOPARTICLES BY CELL-FREE EXTRACT OF Bacillus subtilis

 

Farooqui Maqdoom1, Hashmi Sabeen*2 and Shaikh Zarina3

1. Department of Chemistry, Maulana Azad College, Dr. Rafiq Zakaria Campus, Aurangabad, Maharashtra (INDIA) 2. Department of Biotechnology, Maulana Azad College, Dr. Rafiq Zakaria Campus, Aurangabad, Maharashtra (INDIA) 3. Department of Microbiology. Maulana Azad College, Dr. Rafiq Zakaria Campus, Aurangabad, Maharashtra (INDIA)

 

Received on : August 25, 2014

 

ABSTRACT

 

There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In this paper, we describe a novel combinatorial synthesis approach which is rapid, simple and green for the synthesis of metallic nanostructures of noble metals such as silver (Ag), by using a combination of culture supernatanant of Bacillus subtilis and microwave (MW) irradiation in water. It was found that exposure of culture supernatanant of Bacillus subtilis and microwave irradiation to silver ion lead to the formation of silver nanoparticles. The formation of nanoparticles by this method is extremely rapid and requires no toxic chemicals.

 

Keywords : Bacillus subtilis, Culture supertanant, Microwave irradiation, Silver nanoparticles

 

 

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