Volume No. 9 Issue No.: 2 Page No.: 362-367 October-December 201

 

EFFECT OF 28-HOMOBRASSINOLIDE ON NON-ENZYM- ATIC ANTIOXIDANTS OF TOMATO PLANT UNDER NEMATODE STRESS

 

Kaur Ravinderjit, Ohri Puja*1 and Bhardwaj Renu2

1. Department of Zoology, Guru Nanak Dev University, Amritsar (INDIA) 2. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar (INDIA)

 

Received on : August 10, 2014

 

ABSTRACT

 

In the present investigation, effect of 28-Homobrassinolide (HBl) on non-enzymatic antioxidants of host plant during nematode infection have been evaluated. Surface sterilized seeds of tomato cvs. Pusa Ruby (susceptible) and PNR-7 (resistant) were pre-treated with different concentrations of HBl. Sterilized seeds were then germinated in petri-plates, lined with Whatman sheet no-1 and kept in B.O.D. incubator at 24±2șC. After germination, the seedlings were inoculated with second stage juveniles (J2) of plant-parasitic nematode, Meloidogyne incognita (@5J2/ seedling). The total content of non-enzymatic antioxidants (phenols, flavonoids, ascorbic acid and glutathione) were estimated five days after inoculation (5DAI) in roots and shoots of both the cultivars. Results obtained showed a decrease in total content of non-enzymatic antioxidants with nematode inoculation in susceptible cultivar but an increase in resistant cultivar, but with HBl treatment overall significant increase has been observed. Moreover, when the total antioxidant content of two varieties were compared, overall higher values were found in the resistant cultivar than in the susceptible cultivar. Thus, the investigation suggested a positive role of HBl in activating the immune system of host plant during oxidative stress generated by nematodes.

 

Keywords : 28-Homobrassinolide, Phenol, Flavonoid, Ascorbic acid, Glutathione, Root- knot nematode

 

 

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