Volume No. 9 Issue No.: 2 Page No.: 348-355 October-December 20

 

In vitro PROPAGATION OF Bambusa nutan IN COMMERCIAL SCALE IN ASSAM, INDIA

 

Sharma Pratibha * and Sarma K.P.

Department of Environmental Science, Tezpur University, Napaam, Tezpur, Assam, (INDIA)

 

Received on : August 10, 2014

 

ABSTRACT

 

Standardization of an efficient in vitro propagation of Bambusa nutan for commercial scale with hardening procedure was described in this communication. Successful micropropagation protocol was achieved from field grown culms of B. nutans. Nodes with axillary buds were used as explants to induce multiple shoots on MS (1962) medium. MS medium enriched with BA (1.0 mg/L) supported maximum in vitro shoot multiplication. Sub culturing of shoots were done after every 3 weeks on fresh multiplication medium. The proliferated shoot clusters containing three to five shoots were successfully rooted on MS medium supplemented with IAA (5.0 mg/L)+ IBA (8.0 mg/L)+NAA (5.0 mg/L) resulting in a maximum of 90% rooting. Rooted shoots were semi hardened in root trainer in cocopeat and vermicompost base and kept in poly green house in covered condition for 20-25 days. After that plantlets were fully hardened, acclimatized and established in soil where they exhibited normal and luxuriant growth.

 

Keywords : In vitro propagation, Micro propagation, Shoot multiplication, MS medium, Vermicompost

 

 

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