Volume No. 9 Issue No.: 2 Page No.: 318-323 October-December 201

 

SCREENING OF RHIZOMICROFLORA FROM THE RHI- ZOSPHERE OF Pongamia glabra FOR THEIR PLANT GROWTH PROMOTING AND ANTI -MICROBIAL ACTIVITIES

 

Damle N.R. and Kulkarni S. W.*

1. Department of Microbiology, D.B.F Dayanand college of Arts and Science, Solapur, Maharashtra, (INDIA) 2. Department of Microbiology, S.B.Z. College, Barshi, Maharashtra (INDIA)

 

Received on : September 10, 2014

 

ABSTRACT

 

Rhizosphere is the area of intense microbiological activity. Plant growth promoting rhizomicroflora inhabits rhizosphere of plants and enhance plant growth through production and release of metabolites and also inhibit soil borne plant pathogens by the production of substances like HCN and siderophore. Pongamia glabra (Karanj) is one of the important medicinal plants used to make the oil which is used for pain relief. The present study is intended to isolate plant growth promoting organisms from the rhizosphere of Pongamia glabra. In the present study total 9 bacterial and 5 actinomycetal isolates were obtained. All the isolates were characterized by morphological cultural and biochemical characters. All the isolates were screened for their plant growth promoting activities viz. Ammonia production, HCN production, IAA production, siderophore production and phosphate solubilization and also tested for antimicrobial activity against plant pathogens. The results showed that not all isolates possessed all 5 PGP activities. The % of positive isolates varied. i.e. The 55.5% bacterial isolates showed NH3 production and phosphate solubilization, 66.6% isolates HCN production, and 77.7% isolates showed siderophore production, and no isolate showed IAA production. From 5 actinomycetal isolates 100% showed NH3 and IAA production, and phosphate solubilization 80% isolates showed HCN production and 40% showed siderophore production. Antibacterial and antifungal activity against plant pathogens was observed with PG 5 and PG 7 bacterial isolates and PG4 actinomycete showed inhibition of Fusarium oxysporum. Thus these isolates can be used as sources for plant growth promoting substances and bio controlling agents and hence are agriculturally important.

 

Keywords : Rhizosphere, Pongamia glabra, PGPR, antimicrobial activities, HCNT Siderophere

 

 

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