Volume No. 9 Issue No.: 1 Page No.: 188-191 July-September 2014

 

In vitro EFFECT OF 24 - EPIBRASSINOLIDE ON ANTIOXIDATIVE ENZYMES OF TOMATO PLANTS DURING Meloidogyne incognita INFECTION

 

Jasrotia Shivam and Ohri Puja*

Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab (INDIA)

 

Received on : January 10, 2014

 

ABSTRACT

 

To evaluate the in vitro effect of 24-Epibrassinolide on antioxidative enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione peroxidase, glutathione reductase and superoxide dismutase) of tomato plants during Meloidogyne incognita infection. Uniformly sized, sterilized tomato seeds cv. Pusa ruby (susceptible) were taken and treated with different concentrations of EBl. The seeds were then allowed to germinate in Biological Oxygen Demand (B.O.D.) incubator at 242C. After germination, seeds were inoculated with second stage juveniles (@5J2 plant). The specific activities of antioxidative enzymes were estimated in both roots and shoots 5 days after nematode inoculation (5DAI) using standard protocols. The results determined showed an overall increase in the specific activity of enzymes with EBl treatment. Thus, concluding that EBl application played an important role in boosting the activities of antioxidative enzymes under stress.

 

Keywords : 24-Epibrassinolide, Antioxidative enzymes, BOD, 5DAI, Meloidogyne incognita

 

 

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