Volume No. 9 Issue No.: 1 Page No.: 129-137 July-September 2014

 

BIOREMEDIATION OF DISTILLERY SPENT WASH USING Pseudomonas aeruginosa, Aspergillus niger AND MIXED CONSORTIA

 

Nikam S .B.*1 Saler R. S.2 and Bholay A. D.3

1. Department of Biotechnology, K. T. H. M. College, Nasik, Maharashtra (INDIA)
2. Department of Botany, K. T. H. M. College, Nasik, Maharashtra (INDIA)
3. Department of Microbiology, K.T.H.M. College, Nasik, Maharashtra (INDIA)

 

Received on : December 05, 2013

 

ABSTRACT

 

Distillery spent wash contain melanoidins which are natural condensation products of sugar and amino acids produced by non-enzymatic Millard amino-carbonyl reaction taking place between the amino and carbonyl groups in organic substances. Melanoidins are very important from environmental aspects and due to their structural complexity, dark colour and offensive odor, these pose serious threat to soil and aquatic ecosystem. This causes the problem, like reduction of sunlight penetration, decreased photosynthetic activity and dissolved oxygen concentration in the water bodies whereas on land, it causes reduction in soil alkalinity and inhibition of seed germination. Bioremediation is an ecofriendly technology for treating chemical spills and hazardous waste. It is considered highly desirable to exploit the biodegradation potential of soil microorganisms from polluted sites. Use of microorganisms like Pseudomonas aeruginosa, Aspergillus niger, Streptoccous sp., Bacillus sp. and Staphyloccous aurius will be the cost effective biotechnology for treatment of water polluted by spent wash containing melanoidin. The individual organisms and their mixed consortia degraded the 75% to 80% concentrated spent wash. After the optimization of various physicochemical parameters the mixed consortia exhibited enhanced activity as compared to the individual cultures alone.

 

Keywords : Bioremediation, Melanoidin, Pseudomonas aeruginosa, Aspergillus niger, Consortia, Amino-cubonye rection

 

 

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