Volume No. 9 Issue No.: 1 Page No.: 120-128 July-September 2014

 

DETOXIFICATION OF REACTIVE RED 195 BY Micrococcus glutamicus NCIM 2168

 

Sahasrabudhe Madhuri,* Bhattacharya Aditi,1 Pathade Aparna 2 and Pathade Girish 3

1. Department of Microbiology, Maulana Azad College, Aurangabad, Maharashtra (INDIA)
2. Department of Microbiology, Fergusson College, Pune (INDIA)
3. Department of Biotechnology, Fergusson College, Pune (INDIA)

 

Received on : January 25,2014

 

ABSTRACT

 

Dyes are synthetic recalcitrant aromatic compounds with various functional groups. Reactive azo dyes are considered as one of the most detrimental pollutants from industrial effluents and therefore their biodegradation is receiving constant scientific attention. Micrococcus glutamicus NCIM 2168 was exploited for its ability to degrade Reactive red 195 under static condition. The bacterium took 18 h to decolourize 94.25% of the dye. It decolourized the dye over a wide range of pH from 5-8 and in a narrow temperature from 37-40C. Effect of various carbon and nitrogen sources on decolourization revealed enhanced decolourization in presence of sucrose followed by glucose, yeast extract and peptone. Cell free extract study showed involvement of oxidoreductases enzymes in decolourization. Degradation of the dye was confirmed by Uv-Vis spectroscopy, TLC, HPLC and GC-MS. Microbial toxicity of degraded products confirmed nontoxic nature of the product. The bacterium was also able to degrade mixture of five azo dyes. Operation over a wide range of pH and efficient decolorization of mixture of azo dyes suggested that M.glutamicus NCIM 2168 can be used to decolorize synthetic dyes from effluents.

 

Keywords : Reactive red 195, Azo dyes, Decolourization, Degradation, Oxidoreductases, Microbial toxicity

 

 

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