Volume No. 9 Issue No.: 1 Page No.: 38-49 July-September 2014

 

COMMUNITY COMPOSITION AND POLYCYCLIC AROMATIC HYDROCARBON (PAH) BIODEGRA -DATION POTENTIAL OF MICROORGANISM ISOLATED FROM OILY SLUDGE

 

Das Ranjit, Choudhary Poonam, Oberoi Akashdeep Singh, Dey Priyadarshini and Kazy Sufia K.*

Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur, West Bengal (INDIA)

 

Received on : January 10, 2014

 

ABSTRACT

 

Environmental pollution caused by petroleum and its associated products through accidental or deliberate release results in the calamitous consequences within the ecosystem. Microorganism based bioremediation technology is considered as an efficient, economic and versatile alternative to conventional treatment methods for the reclamation of oil contaminated sites. This study was conducted to assess the genetic diversity and catabolic potential of indigenous microorganism in a petroleum hydrocarbon contaminated sludge sample obtained from an oil storage facility. Twenty eight (28) indigenous bacterial strains were isolated from the oily sludge. Most of these strains were identified as gram-positive bacteria that were phylogenetically affiliated to the genera Bacillus (more than 46%), Paenibacillus (~11%), Micrococcus (7%), Brachybacterium (7%), Aerococcus (14%) and Zimmermannella. Among the gram-negative strains Pseudomonas sp. and Pseudoxanthomonas sp. were identified. One of these bacteria, Aerococcus sp. strain BPD- 6 was further characterized in terms of its heavy metal resistance and other hydrocarbon utilization potential. Naphthalene and anthracene degradation potential of Aerococcus sp. BPD-6 was studied over a wide concentration range and the kinetic parameters were estimated using four growth kinetic models. Overall observation indicated that the strain may be useful for bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs).

 

Keywords : Aerococcus, Bioremediation, PAH, Kinetic parameters sludge

 

 

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