Volume No. 9 Issue No.: 1 Page No.: 15-23 July-September 2014

 

MOLECULAR SURVEY ON BACTERIAL DIVERSITY IN ARSENIC CONTAMINATED SUBSURFACE SEDIMENT IN WEST BENGAL, INDIA

 

Paul Dhiraj, Sar Pinaki*1 Kazy Sufia K.2 and Pal Taraknath3

1. Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal (INDIA)
2. Department of Biotechnology, National Institute of Technology, Durgapur, West Bengal (INDIA)
3. Central Headquarters, Geological Survey of India, Kolkata, West Bengal (INDIA)

 

Received on : December 20,2013

 

ABSTRACT

 

The present work investigated indigenous bacterial community composition within a highly contaminated aquifer zone of West Bengal, India. Culture independent method of Denaturing Gradient Gel Electrophoresis (DGGE) targeting 16S rRNA genes recovered from sediments collected from subsurface drilling was used to elucidate the bacterial community composition. DGGE based microbial community analysis of subsurface sediments was carried out for varying depths and hydrogeological settings. Presence of iron reducing Geobacter, gram positive high GC content Bacillus and C1 compound utilizing Methylophilaceae bacteria were detected along with the members of Firmicutes, Cyanobacteria and uncultured Bacteroidetes. The cluster analysis reveals most similar and possibly near similar type of microbial diversity in As-6 (grey fine sand) and As-1 (grey soft silt) samples. Also similar type community structure exists in As-7 (orange white coarse sand) and As-9 (orange sand) samples. Samples of As-5 (grey fine sand), As-2 (grey fine sand) and As-4 (grey fine sand) also share similar community structures. Detailed bacterial analysis of these subsurface samples would thus help in framing up a model for biogeochemical release of arsenic in specific microenvironments.

 

Keywords : Arsenic, Bacterial diversity, DGGE, Cyanobacteria, Gel-electrophoeresis

 

 

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