Volume No. 8 Issue No.: 4 Page No.: 895-902 April-June 2014

 

In vitro PROPAGATION OF Bambusa pallida ON COMMERCIAL SCALE IN ASSAM, INDIA

 

Sharma Pratibha* and Sarma K.P.

Department of Environmental Science, Tezpur University, Tezpur, Assam (INDIA)

 

Received on : December 08, 2013

 

ABSTRACT

 

A complete protocol for in vitro propagation of Bambusa pallida is described in this communication. In vitro multiplication was achieved from nodal explants containing axillary buds from field grown culms of Bambusa pallida. Highest multiple shoots were induced on Murashige and Skoog’s medium supplemented with 1.0 mg/l 6-Benzyle Adenine (BA). Proliferating shoot cultures were established by repeatedly subculturing shoots in propagules of 3 shoots each for maintaining a stock of mother culture. Roots were formed on excised propagules of 3 shoots when transferred to MS medium with Indole-3-Acetic Acid (IAA), Indole-3- Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA). Response of rooting was found more in 5.0 mg/l naphthalene acetic acid. Rooted plantlets were successfully hardened, acclimatized and established in soil, where they exhibited normal growth. The survival rate was recorded 100 percent in field condition.

 

Keywords : In vitro propagation, Multiple shoots, Nodal explants, Bamboo, Micropropagation

 

 

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