Volume No. 8 Issue No.: 4 Page No.: 876-882 April-June 2014

 

EVALUATION AND CHARACTERIZATION OF HEAVY METAL RESISTANT FUNGI FOR THEIR PROSPECTS IN BIOREMEDIATION

 

Joshi B. H.

Department of Food Quality Assurance, College of Food Processing Technology and Bioenergy, Anand Agricultural University, Gujarat (INDIA)

 

Received on : January 20, 2014

 

ABSTRACT

 

Fungi executes major role in heavy metals biosorption from contaminated soil. The concentration of heavy metals is increasing due to rapid industrialization and urbanization. Heavy metals having relatively high density are toxic at low concentration. Developing new bioremediation processes for contaminated soils polluted by heavy metals is one the growing interest. The study was conducted to screen out fungal resistance to heavy metals such as lead, nickel, cobalt, chromium, copper, manganese and zinc. The results shows that SG1 isolate obtained during screening with maximum threshold level of tolerance to manganese (60 mg/ml), copper (5 mg/ml), cobalt (5 mg/ml), lead (4.5 mg/ml), nickel (6 mg/ml), chromium (3.9 mg/ml)while isolate GK1 was found to have maximum threshold level of tolerance to zinc (80 mg/ml). Maximum growth of the isolate SG1 at threshold concentrations of metals was found at pH 5 and temperature 30°C in presence of manganese, pH 5 and 32°C with copper, pH 5 and 30°C with cobalt, pH 5 and 31°C with lead, pH 5 and 30°C with nickel, pH 5 and 29°C with chromium. Isolate GK1 showed maximum growth at pH 4 and 30°C with zinc. The results also reveal that antibiotic resistance and metal resistance were highly coupled. Isolate GK1 was found to have multiple antibiotic resistances. UV mutagenesis of isolate GK1 was found to improve the resistance against Zn up to 82 mg/ml. Based on the morphological characterization, it was found that the isolates SG1 and GK1 were of Neurospora sp. and Penicillium sp. respectively.

 

Keywords : Bioremediation, Heavy metal resistance, Antibiotics, UV mutagenesis, Neurospora sp., Penicillium sp.

 

 

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