Volume No. 8 Issue No.: 4 Page No.: 859-866 April-June 2014

 

DETERMINATION OF ORGANOPHOSPHORUS PESTICIDE RESIDUES IN WHEAT AND RICE BY QuEChERS METHOD

 

Trivedi Purushottam1,2, Sharma V. P., Srivastava L.P.1 and Malik Sarika*2

1. CSIR-Indian Institute of Toxicology Research, Lucknow (INDIA)
2. Department of Chemistry, School of Sciences, Noida International University, Noida (INDIA)

 

Received on : January 03, 2014

 

ABSTRACT

 

Cereals are an essential component of human diet. Pesticides constitute an important component in agriculture development and protection of public health in India since the tropical climate is very conductive to pest breeding. Several hundred pesticides of different chemical nature are currently used for agricultural purposes all over the world. QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation was used for the determination of 16 Organophosphate Pesticide Residues (OPPs) in food grain samples, wheat and cereal collected from local market of Lucknow city, India. The pesticide residues analysis method was based on QuEChERS in which the best approach to extract with ethyl acetate followed by clean-up of extracts using anhy.MgSO4 and Primary Secondary Amine (PSA). The analyses were carried out with gas-chromatography (GCNPD/MS). The method was standardized in samples spiked at 0.10 and 0.50 mg/kg and the average recovery varied from 70.20% to 95.25% with Relative Standard Deviation (RSDs) < 15%. The method showed Limit Of Detection (LODs) ranged from 0.001 to 0.010 mg/kg Out of 16 OPPs only chlorofenvinfos and ethion were detected above Maximum Residues Limit (MRL).

 

Keywords : OPPs, QuEChERS, Chromatography, Tropical climate, MRL, LODs

 

 

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