Volume No. 7 Issue No.: 4A Page No.: 1539-1546 April-June 2013

 

OPTIMIZATION OF PHYSIOLOGICAL PARAMETERS FOR PECTINASE PRODUCTION FROM SOIL ISOLATES AND ITS APPLICATIONS IN FRUIT JUICE CLARIFICATION

 

Mehta S.A.*, Rana Mitali, Shaikh Nilofer and Patel Nimisha

Department of Biotechnology and Microbiology, C.G. Bhakta Institute of Biotechnology,
Bardoli-Mahuva Road, Surat, (INDIA)

 

Received on : December 07, 2012

 

ABSTRACT

 

Pectinase enzymes are today one of the upcoming enzyme of commercial sector. It has been reported that microbial pectinase account for 25% of global food sales. Primarily, these enzymes are heterogeneous group of enzymes that hydrolyse the pectin substance, present mostly in plants. It has also been used to increase yields and clarify fruit juices. Pectinases are one of the most widely distributed enzymes in bacteria, fungi and plants. The present investigation was undertaken to isolate pectinase producing microorganism and to standardize the condition for cost effective production of enzyme. Total 15 samples were collected from different sites and 47 isolates were obtained. From this 47 different isolates, 12 isolates were efficient producers. Among them FW5 produced maximum zone of clearance of 3.2 mm. FW2, L4, L3,GF13,GF14,GF12,L1 have more pectinolytic potential. GF1, GF10, MW8, G1 were less capable for pectin degradation. All those 12 isolates were then analysed for secondary screening.Enzyme productivity (EU/ml) and range for pectin degradation was calculated. FW2 had maximum capacity as, enzyme yield of it is high (22 EU/ml).L6 and FW5 were efficient for enzyme production and their enzyme yield were 17.1 and 19 EU/ml. All 12 isolates were tentatively identified following Bergey’s Manual of determinative bacteriology. After characterization FW5 isolate was identified as Bacillus sp. FW2 isolate as Erwinia sp. Other isolates could not be identified specifically.Optimization of temperature, pH,pectin concentration and time was done.Application in fruit juice clarification was found.

 

Keywords : Microbial pectinase production, Enrichment, Isolation, Screening, Pectinase assay

 

 

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